Ribosomal protein L2



Ribosomal protein L2 (RPL2) is one of the 34 proteins which belong to the large 50S subunit of the prokaryotic ribosome or to the ca. 49 proteins in the large 60S subunit of the eukaryotic ribosome. It is required for the association of the 30S and 50S subunits and is involved in the binding of tRNA at the A- and P-sites. In the archaea like in Haloarcula marismortui, the L2 is called L2P (RPL2P). The images at the left and at the right correspond to one representative RPL2, i.e. the crystal structure of Ribosomal protein L2 from Thermus thermophilus (2xg0, chain D).

3D Structures of Ribosomal protein L2
Update June 2011

RPL2
1rl2 – RPL2 – Geobacillus stearothermophilus

L2 in 50S subunit+RNA
2xg0, 2xg2, 3kir, 3kit, 3kiw, 3kiy – TtRPL2 in 50S subunit+RNA fragments – Thermus thermophilus

2x9s, 2x9u, 3kni, 3knk, 3knm, 3kno, 2wrj, 2wrl, 2wro, 2wrr, 3hux, 3huz, 2wh2, 2wh4, 2wdi, 2wdj, 2wdl, 2wdn, 1vsp, 2v47, 2v49, 1vsa, 2hgj, 2hgq, 2hgu, 2j01, 2j03, 1yl3, 3mrz, 3ms1, 3i8f, 3i8i, 3i9c, 3i9e, 3f1f, 3f1h, 3d5b, 3d5d, 2b66, 2b9n, 2b9p, 1giy, 2y0v, 2y0x, 2y0z, 2y11, 2y13, 2y15, 2y17, 2y19, 3pyo, 3pyr, 3pyt, 3pyv - TtRPL2 in 50S subunit+5S RRNA+23S RRNA

3fin - TtRPL2 in 50S subunit+5S RRNA+23S RRNA – Cryo-EM

2wwq – EcRPL2 in 50S subunit+5S RRNA+23S RRNA+mRNA+P-site tRNA – Escherichia coli

3i1n, 3i1p, 3i1r, 3i1t, 3i20, 3i22, 3e1b, 3e1d, 3df2, 3df4, 2vhn, 2qov, 2qox, 2i2t, 2i2v, 1vs6, 1vs8, 2aw4, 2awb, 3ofd, 3og0, 1vor, 1vou, 1vow, 1voy, 1pnu, 1pny,3r8s, 3r8t - EcRPL2 in 50S subunit+5S RRNA+23S RRNA

3fik, 2gya, 2gyc, 3bbx, 1p85, 1p86, 3izt, 3izu -  EcRPL2 in 50S subunit+5S RRNA+23S RRNA – Cryo-EM

3kcr - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+preprotein translocase

3j01 - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+preprotein translocase –Cryo-EM

2rdo - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+EF-G+RRF – Cryo-EM

3bbo - RPL2 in 50S subunit+5S RRNA+23S RRNA+4.8S RRNA – Cryo-EM – spinach

3iy9 – RPL2 in 50S subunit – Leishmania taretolae

2zjq, 2zjr, 1nkw - DrRPL2 in 50S subunit+5S RRNA+23S RRNA – Deinococcus radiodurans

1kqs, 1qvg - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+CCA - Haloarcula marismortui

1jj2, 1ffk, 3cc2, 2qa4, 1s72 - HmRPL2P in 50S subunit+5S RRNA+23S RRNA

3cc7, 3cce, 3ccj, 3ccl, 3ccm, 3ccq, 3ccr, 3ccs, 3ccu, 3ccv - HmRPL2P in 50S subunit+5S RRNA+23S RRNA (mutant)

1vq4, 1vq5, 1vq7, 1vql, 1vqm, 1vqp - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+transition state analog

1vq6, 1vqk, 1vqn - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+ c-hpmn+CCA-Phe-caproic acid-biotin

1vqo - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+ CCPMN

1yhq, 1yi2, 1yij, 1yjn, 1yjw - HmRPL2P in 50S subunit+5S RRNA+23S RRNA (mutant)+antibiotic

1yj9 - HmRPL2P in 50S subunit (L22 mutant)+5S RRNA+23S RRNA

1qvf - HmRPL2P in 50S subunit+5S RRNA+23S RRNA + deacylated tRNA minihelix

1q86 - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+CCA-Phe-caproic acid-biotin

L2 in 50S subunit + antibiotics
3dll, 2zjp, 3cf5, 1xbp, 1sm1, 1nwx, 1nwy, 3pio, 3pip - DrRPL2 in 50S subunit+5S RRNA+23S RRNA+antibiotic

3oh5, 3oh7, 3ohj, 3ohk, 3ohz, 3oi1, 3oi3, 3oi5 - TtRPL2 in 50S subunit+5S RRNA+23S RRNA+antibiotic

2qam, 2qao, 2qoz, 2qp1, 2qba, 2qbc, 3ofc, 3ofz, 3ofq, 3ofr, 1vt2, 3orb, 3oas, 3oat -  EcRPL2 in 50S subunit+5S RRNA+23S RRNA+antibiotic

1k73, 1kc8, 1k8a, 1k9m, 1kd1, 1m1k, 3i55, 3i56, 3g4s, 3g6e, 3g71, 2qex, 3cpw, 3cc4, 3cd6, 2otj, 2otl, 1yit, 1q7y, 1q81, 1q82, 1n8r, 1nji, 3cxc - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+antibiotics

1m90, 3cma, 3cme, 1vq8, 1vq9 - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+ sparsomycin+CCA-Phe-caproic acid-biotin

L2 in 60S subunit
3jyw - RPL2 in 60S subunit – Thermomyces lanuginosus – Cryo-EM

3izr - RPL2 in 60S subunit – wheat – Cryo-EM

2ftc - RPL2 in 60S subunit+16S RRNA - bovine

1s1i - yRPL2 in 40S ribosomal subunit+18S RRNA+elongation factor 2 – Cryo-EM – yeast

3o58, 3o5h - yRPL2 in 60S subunit+25S RRNA+5SRRNA+5.8RRNA

L2 in part of the large ribosomal subunit
487d – RPL2+RPL1+RPL6+RPL9+RPL11+RPL14+RPL25 - Cryo-EM

1c04 – HmRPL2P+RPL6+RPL11+RPS14+23S RRNA fragment

L2 in large + small ribosomal subunit
1ml5 - EcRPL2 in 50S ribosomal subunit+30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA – EM

L2 in 50S subunit + ribosome releasing factor (RRF)
2qbe, 2qbg, 2qbi, 2qbk, 2z4l, 2z4n - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+RRF

L2 in 50S subunit + various proteins
2vhm - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+PDF C-terminal helix

2j28 - EcRPL2 in 50S subunit+5S RRNA+23S RRNA+signal recognition particle – Cryo-EM

1w2b - HmRPL2P in 50S subunit+5S RRNA+23S RRNA+trigger factor N-terminal